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An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers

Figure 3

Chloroplast DNA visualization of Araucaria angustifolia in 0.7% agarose gel stained with ethidium bromide.

(A) Ladder 1 kb and cpDNA isolated with modified high salt method; (B) Ladder 1 kb and cpDNA isolated with sucrose method; (C) Ladder 1 kb and cpDNA isolated with high salt plus saline Percoll method.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0084792.g003