An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers
Figure 2
Chloroplast visualization of Araucaria angustifolia in phase contrast microscopy.
(A) Chloroplasts isolated with improved high salt method; (B) Chloroplasts isolated with sucrose method; (C) Chloroplasts isolated with high salt plus Percoll method; (D–F) Micrographs during chloroplast isolation with high salt plus saline Percoll method; (D) Broken and intact chloroplasts before Percoll gradient centrifugation; (E) Intact isolated chloroplasts in interface 70/30% after Percoll gradient centrifugation; (F) Broken chloroplasts in upper 30% phase after Percoll gradient centrifugation. Dotted arrows indicate broken chloroplasts. Solid arrows indicate intact chloroplasts. Bar –50 µM.