Chromatin Modification by PSC Occurs at One PSC per Nucleosome and Does Not Require the Acidic Patch of Histone H2A
Figure 6
(a) Schematic diagram of DNA bridging assay. (b) Representative analysis of bridging of naked DNA by PSC. Top panels show sucrose gradient fractions that were pooled for streptavidin pull-down. Bottom panels show streptavidin pull-down results. The per cent bound refers to how much of the unbiotinylated fragment is present in the pellet as a fraction of the total (pellet + supernatant). Asterisks indicate position of biotinylated fragment; note that in pellet fractions, biotinylated fragment is incompletely recovered by Proteinase K treatment of streptavidin coated beads, and migrates slowly likely due to bound streptavidin. (c) Summary of streptavidin pull-down experiments. Graphs show average per cent of the unbiotinylated fragment associated with streptavidin beads.