An Efficient Approach for the Development of Locus Specific Primers in Bread Wheat (Triticum aestivum L.) and Its Application to Re-Sequencing of Genes Involved in Frost Tolerance

Open Access

Peer-reviewed

Research Article

Steve Babben,

Affiliation Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Sachsen-Anhalt, Germany
⨯
Dragan Perovic ,

* E-mail: dragan.perovic@jki.bund.de

Affiliation Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Sachsen-Anhalt, Germany
⨯
Michael Koch,

Affiliation Deutsche Saatveredelung AG (DSV), Lippstadt, Nordrhein-Westfalen, Germany
⨯
Frank Ordon

Affiliation Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Sachsen-Anhalt, Germany
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Wheat
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Polymerase chain reaction
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Common wheat
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Nucleotide sequencing
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Genomics
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Chromosome mapping
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BLAST algorithm
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Gene sequencing
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