Gene Targeting in the Red Alga Cyanidioschyzon merolae: Single- and Multi-Copy Insertion Using Authentic and Chimeric Selection Markers

• Takayuki Fujiwara,

  Current address: National Institute of Genetics, Mishima, Japan

  Affiliation Chromosome Dynamics Laboratory, RIKEN, Wako, Saitama, Japan

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• Mio Ohnuma,

  Affiliations Faculty of Science, Rikkyo University, Toshima-ku, Tokyo, Japan, Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Chiyoda-ku, Tokyo, Japan

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• Masaki Yoshida,

  Affiliation Faculty of Life & Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan

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• Tsuneyoshi Kuroiwa,

  Affiliations Faculty of Science, Rikkyo University, Toshima-ku, Tokyo, Japan, Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Chiyoda-ku, Tokyo, Japan

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• Tatsuya Hirano

  * E-mail: hiranot@riken.jp

  Affiliation Chromosome Dynamics Laboratory, RIKEN, Wako, Saitama, Japan

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