Measurement of the Infection and Dissemination of Bluetongue Virus in Culicoides Biting Midges Using a Semi-Quantitative RT-PCR Assay and Isolation of Infectious Virus

• Eva Veronesi,

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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• Frank Antony,

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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• Simon Gubbins,

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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• Nick Golding,

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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• Alison Blackwell,

  Affiliation Advanced Pest Solutions Ltd., Dundee, United Kingdom

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• Peter PC. Mertens,

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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• Joe Brownlie,

  Affiliation Royal Veterinary College, Hatfield, Herts, United Kingdom

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• Karin E. Darpel,

  Affiliations Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom, University of Surrey, Guildford, Surrey, United Kingdom

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• Philip S. Mellor,

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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• Simon Carpenter

  * E-mail: simon.carpenter@pirbright.ac.uk

  Affiliation Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, United Kingdom

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