Characterization of Arabidopsis FPS Isozymes and FPS Gene Expression Analysis Provide Insight into the Biosynthesis of Isoprenoid Precursors in Seeds

Verónica Keim; David Manzano; Francisco J. Fernández; Marta Closa; Paola Andrade; Daniel Caudepón; Cristina Bortolotti; M. Cristina Vega; Montserrat Arró; Albert Ferrer

doi:10.1371/journal.pone.0049109

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Peer-reviewed

Research Article

Characterization of Arabidopsis FPS Isozymes and FPS Gene Expression Analysis Provide Insight into the Biosynthesis of Isoprenoid Precursors in Seeds

Verónica Keim,

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
David Manzano,

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
Francisco J. Fernández,

Affiliation Department of Structural and Quantitative Biology, Centre for Biological Research (CIB-CSIC), Madrid, Spain
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Marta Closa,

Affiliation Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
Paola Andrade,

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
Daniel Caudepón,

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
Cristina Bortolotti,

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
M. Cristina Vega,

Affiliation Department of Structural and Quantitative Biology, Centre for Biological Research (CIB-CSIC), Madrid, Spain
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Montserrat Arró ,

Contributed equally to this work with: Montserrat Arró, Albert Ferrer

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯
Albert Ferrer

Contributed equally to this work with: Montserrat Arró, Albert Ferrer

* E-mail: albertferrer@ub.edu

Affiliations Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès), Barcelona, Spain, Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
⨯

Characterization of Arabidopsis FPS Isozymes and FPS Gene Expression Analysis Provide Insight into the Biosynthesis of Isoprenoid Precursors in Seeds

Verónica Keim,
David Manzano,
Francisco J. Fernández,
Marta Closa,
Paola Andrade,
Daniel Caudepón,
Cristina Bortolotti,
M. Cristina Vega,
Montserrat Arró,
Albert Ferrer

Published: November 7, 2012
https://doi.org/10.1371/journal.pone.0049109

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Arabidopsis thaliana
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Seeds
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Isozymes
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Isoprenoids
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Gene expression
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Seedlings
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Protein extraction
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