Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos

Mario Hermann; Morgan L. Maeder; Kyle Rector; Joseph Ruiz; Burkhard Becher; Kurt Bürki; Cyd Khayter; Adriano Aguzzi; J. Keith Joung; Thorsten Buch; Pawel Pelczar

doi:10.1371/journal.pone.0041796

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Open Access

Peer-reviewed

Research Article

Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos

Mario Hermann,

Affiliations Institute of Laboratory Animal Science, University of Zurich, Zurich, Switzerland, Institute of Neuropathology, University Hospital Zurich, Zurich, Switzerland
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Morgan L. Maeder,

Affiliation Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
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Kyle Rector,

Affiliation Transposagen Biopharmaceuticals, Inc., Lexington, Kentucky, United States of America
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Joseph Ruiz,

Affiliation Transposagen Biopharmaceuticals, Inc., Lexington, Kentucky, United States of America
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Burkhard Becher,

Affiliation Institute for Experimental Immunology, University of Zurich, Zurich, Switzerland
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Kurt Bürki,

Affiliation Institute of Laboratory Animal Science, University of Zurich, Zurich, Switzerland
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Cyd Khayter,

Affiliation Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
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Adriano Aguzzi,

Affiliation Institute of Neuropathology, University Hospital Zurich, Zurich, Switzerland
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J. Keith Joung,

Affiliations Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America, Department of Pathology, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Thorsten Buch ,

Contributed equally to this work with: Thorsten Buch, Pawel Pelczar

* E-mail: thorsten.buch@mikrobio.med.tum.de (TB); pawel.pelczar@ltk.uzh.ch (PP)

Affiliations Institute for Experimental Immunology, University of Zurich, Zurich, Switzerland, Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, Munich, Germany
⨯
Pawel Pelczar

Contributed equally to this work with: Thorsten Buch, Pawel Pelczar

* E-mail: thorsten.buch@mikrobio.med.tum.de (TB); pawel.pelczar@ltk.uzh.ch (PP)

Affiliation Institute of Laboratory Animal Science, University of Zurich, Zurich, Switzerland
⨯

Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos

Mario Hermann,
Morgan L. Maeder,
Kyle Rector,
Joseph Ruiz,
Burkhard Becher,
Kurt Bürki,
Cyd Khayter,
Adriano Aguzzi,
J. Keith Joung,
Thorsten Buch

Published: September 6, 2012
https://doi.org/10.1371/journal.pone.0041796

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Non-homologous end joining
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Zygotes
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Zinc finger nucleases
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Embryos
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Mammalian genomics
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Microinjection
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Gene targeting
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Genetic loci
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