Structuring Detergents for Extracting and Stabilizing Functional Membrane Proteins

Rima Matar-Merheb; Moez Rhimi; Antoine Leydier; Frédéric Huché; Carmen Galián; Elodie Desuzinges-Mandon; Damien Ficheux; David Flot; Nushin Aghajari; Richard Kahn; Attilio Di Pietro; Jean-Michel Jault; Anthony W. Coleman; Pierre Falson

doi:10.1371/journal.pone.0018036

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Peer-reviewed

Research Article

Structuring Detergents for Extracting and Stabilizing Functional Membrane Proteins

Rima Matar-Merheb,

Affiliation CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France
⨯
Moez Rhimi ,

Contributed equally to this work with: Moez Rhimi, Antoine Leydier, Frédéric Huché

Affiliations CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France, Laboratoire de BioCristallographie et Biologie Structurale des Cibles Thérapeutiques, Université Lyon 1, Univ Lyon; CNRS, UMR 5086; Bases Moléculaires et Structurales des Systèmes Infectieux, IBCP, Lyon, France
⨯
Antoine Leydier ,

Contributed equally to this work with: Moez Rhimi, Antoine Leydier, Frédéric Huché

Affiliation CEA DEN/DRCP/SCPS/LEPS, Bagnols-sur-Cèze, France
⨯
Frédéric Huché ,

Contributed equally to this work with: Moez Rhimi, Antoine Leydier, Frédéric Huché

Affiliations CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France, Laboratoire de BioCristallographie et Biologie Structurale des Cibles Thérapeutiques, Université Lyon 1, Univ Lyon; CNRS, UMR 5086; Bases Moléculaires et Structurales des Systèmes Infectieux, IBCP, Lyon, France
⨯
Carmen Galián,

Affiliation CNRS/CEA/Université Joseph Fourier, Institut de Biologie Structurale, Grenoble, France
⨯
Elodie Desuzinges-Mandon,

Affiliation CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France
⨯
Damien Ficheux,

Affiliation CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France
⨯
David Flot,

Affiliation European Synchrotron Radiation Facility, BP 220, Grenoble, France
⨯
Nushin Aghajari,

Affiliation Laboratoire de BioCristallographie et Biologie Structurale des Cibles Thérapeutiques, Université Lyon 1, Univ Lyon; CNRS, UMR 5086; Bases Moléculaires et Structurales des Systèmes Infectieux, IBCP, Lyon, France
⨯
Richard Kahn,

Affiliation CNRS/CEA/Université Joseph Fourier, Institut de Biologie Structurale, Grenoble, France
⨯
Attilio Di Pietro,

Affiliation CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France
⨯
Jean-Michel Jault,

Affiliation CNRS/CEA/Université Joseph Fourier, Institut de Biologie Structurale, Grenoble, France
⨯
Anthony W. Coleman ,

* E-mail: p.falson@ibcp.fr (PF); anthony.coleman@univ-lyon1.fr (AWC)

Affiliation Laboratoire des Multimatériaux et Interfaces, UMR 5615 CNRS/Université de Lyon 1, Lyon, France
⨯
Pierre Falson

* E-mail: p.falson@ibcp.fr (PF); anthony.coleman@univ-lyon1.fr (AWC)

Affiliation CNRS, Drug Resistance Mechanism and Modulation Laboratory, Ligue labeled team 2009, Lyon, France
⨯

Structuring Detergents for Extracting and Stabilizing Functional Membrane Proteins

Rima Matar-Merheb,
Moez Rhimi,
Antoine Leydier,
Frédéric Huché,
Carmen Galián,
Elodie Desuzinges-Mandon,
Damien Ficheux,
David Flot,
Nushin Aghajari,
Richard Kahn

Published: March 31, 2011
https://doi.org/10.1371/journal.pone.0018036

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Membrane proteins
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Protein extraction
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Adenosine triphosphatase
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