Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

Delyan P. Ivanov; Terry L. Parker; David A. Walker; Cameron Alexander; Marianne B. Ashford; Paul R. Gellert; Martin C. Garnett

doi:10.1371/journal.pone.0103817

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Open Access

Peer-reviewed

Research Article

Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

Delyan P. Ivanov,

Affiliation School of Pharmacy, University of Nottingham, Nottingham, United Kingdom
⨯
Terry L. Parker,

Affiliation Medical School, Queens Medical Centre, University of Nottingham, Nottingham, United Kingdom
⨯
David A. Walker,

Affiliation Children's Brain Tumour Research Centre, Queens Medical Centre, University of Nottingham, Nottingham, United Kingdom
⨯
Cameron Alexander,

Affiliation School of Pharmacy, University of Nottingham, Nottingham, United Kingdom
⨯
Marianne B. Ashford,

Affiliation AstraZeneca, Macclesfield, United Kingdom
⨯
Paul R. Gellert,

Affiliation AstraZeneca, Macclesfield, United Kingdom
⨯
Martin C. Garnett

* E-mail: martin.garnett@nottingham.ac.uk

Affiliation School of Pharmacy, University of Nottingham, Nottingham, United Kingdom
⨯

Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

Delyan P. Ivanov,
Terry L. Parker,
David A. Walker,
Cameron Alexander,
Marianne B. Ashford,
Paul R. Gellert,
Martin C. Garnett

Published: August 13, 2014
https://doi.org/10.1371/journal.pone.0103817

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Protocols and updated macro

Posted by divanov on 14 Jun 2017 at 09:57 GMT

The protocols for the assays in this manuscript have now been published by Springer:
https://doi.org/10.1007/9...

I have also developed a new version of the spheroid size macro which works on whole folders of images and image stacks:
https://figshare.com/s/99...

Example images used in the second generation macro can be found here:
https://figshare.com/s/ad...

No competing interests declared.

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Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

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Protocols and updated macro

Posted by divanov on 14 Jun 2017 at 09:57 GMT