About the Authors

Amanda L. Evans

Affiliations Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom, Medical Research Council, National Institute for Medical Research, London, United Kingdome

Tiago Faial

Affiliations The Anne McLaren Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, United Kingdom, Department of Zoology, University of Cambridge, Cambridge, United Kingdom, Medical Research Council, National Institute for Medical Research, London, United Kingdome

Michael J. Gilchrist

Affiliations Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom, Medical Research Council, National Institute for Medical Research, London, United Kingdome

Thomas Down

Affiliation Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom

Ludovic Vallier

Affiliation The Anne McLaren Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, United Kingdom

Roger A. Pedersen

Affiliation The Anne McLaren Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, United Kingdom

Fiona C. Wardle

Affiliation Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom

James C. Smith

jim.smith@nimr.mrc.ac.uk

Affiliations Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom, Department of Zoology, University of Cambridge, Cambridge, United Kingdom, Medical Research Council, National Institute for Medical Research, London, United Kingdome

Competing Interests

A proportion of the consumables funding for the RAP laboratory during the period in which the work was accomplished was provided by PluriSys. PluriSys is a non-commercial, FP7 consortium grant funded by the European Commission. BioTalentum, a Hungarian limited company, coordinates the PluriSys consortium but does not take any commercial interest in the materials or results generated by the consortium. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: ALE TF RAP LV FCW JCS. Performed the experiments: ALE TF. Analyzed the data: ALE TF FCW MJG TD JCS. Contributed reagents/materials/analysis tools: LV RAP. Wrote the paper: ALE TF RAP FCW JCS.