Figures
Necator americanus the "American Murderer"
Controlled exsheathment of axenic Necator americanus L3 larvae was harnessed to prepare partially exsheathed hookworms, which were analysed using Atomic Force Microscopy (AFM) and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). AFM revealed previously unseen surface area enhancing nano-annuli exclusive to the sheath surface. ToF-SIMS elucidated unique and significantly different chemistries between the surface of the emergent cuticle (lubricating phosphatidylglycerol-like-head groups, red) and deposited sheath (immunogenic heparan sulphate-like-monosaccharides, green). Chauhan et al.
Image Credit: Dr Veeren M Chauhan
Citation: (2017) PLoS Neglected Tropical Diseases Issue Image | Vol. 11(12) December 2017. PLoS Negl Trop Dis 11(12): ev11.i12. https://doi.org/10.1371/image.pntd.v11.i12
Published: December 29, 2017
Copyright: © 2017 Chauhan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Controlled exsheathment of axenic Necator americanus L3 larvae was harnessed to prepare partially exsheathed hookworms, which were analysed using Atomic Force Microscopy (AFM) and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). AFM revealed previously unseen surface area enhancing nano-annuli exclusive to the sheath surface. ToF-SIMS elucidated unique and significantly different chemistries between the surface of the emergent cuticle (lubricating phosphatidylglycerol-like-head groups, red) and deposited sheath (immunogenic heparan sulphate-like-monosaccharides, green). Chauhan et al.
Image Credit: Dr Veeren M Chauhan