Figures
The helminth fluorescent bioassay: An objective tool for quantifying schistosome viability.
Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.
Image Credit: Emily Peak
Citation: (2010) PLoS Neglected Tropical Diseases Issue Image | Vol. 4(7) July 2010. PLoS Negl Trop Dis 4(7): ev04.i07. https://doi.org/10.1371/image.pntd.v04.i07
Published: July 27, 2010
Copyright: © 2010 Emily Peak. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.
Image Credit: Emily Peak