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PLoS Neglected Tropical Diseases Issue Image | Vol. 2(6) June 2008

Leishmania chagasi-Infected Human Monocyte-Derived Macrophages Co-Cultured with Autologous Leishmania-Naïve T Cells

Human monocyte-derived macrophages from a Leishmania-naïve donor were infected with Leishmania chagasi promastigote for 24 hrs, and co-cultured with purified CD3+ autologous T cells for an additional 24 hours. Cells were fixed and stained with Diff Quik. Images (600x) show an uninfected macrophage and several infected macrophages with intracellular amastigotes. Numerous T cells are visible in the image (see Ettinger and Wilson, doi:10.1371/journal.pntd.0000252).

Image Credit: Image generated by Nicholas A. Ettinger.

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Leishmania chagasi-Infected Human Monocyte-Derived Macrophages Co-Cultured with Autologous Leishmania-Naïve T Cells

Human monocyte-derived macrophages from a Leishmania-naïve donor were infected with Leishmania chagasi promastigote for 24 hrs, and co-cultured with purified CD3+ autologous T cells for an additional 24 hours. Cells were fixed and stained with Diff Quik. Images (600x) show an uninfected macrophage and several infected macrophages with intracellular amastigotes. Numerous T cells are visible in the image (see Ettinger and Wilson, doi:10.1371/journal.pntd.0000252).

Image Credit: Image generated by Nicholas A. Ettinger.

https://doi.org/10.1371/image.pntd.v02.i06.g001