Table 1.
Demographics based on Sire’s Leishmania diagnostic status. Sire’s ever positive diagnostic status was determined as sire ever positive via qPCR or serology.
Table 2.
Univariate analysis of risk factors for impact of sire’s infection on transmission to Pup. Pup ever diagnostically positive is the outcome with sire’s ever positive diagnostic status as the exposure. Sire’s ever positive diagnostic status was determined as sire ever positive via qPCR or serology during the year of birth. Number of pups with missing sire diagnostics: 111, 132, 263, 284, 375, and 416. + Diagnostic status during year of birth.
Table 3.
Binomial regression with log-link (RR) analysis for risk factors associated with impact of sire’s on Leishmania venereal transmission. Model A: Explanatory variables include overall diagnostic status of the sire (never diagnostically positive compared to ever diagnostically positive), the sire’s age during the year of birth (younger or equal to 7 years of age compared to older than 7 years of age), and the sex of the puppy (female compared to male). Model B: Explanatory variables are sire’s diagnostic status at the year of pup’s birth, the sire’s age during the year of birth, and sex of the puppy. Model C: Explanatory variables include sire’s qPCR and serology statuses and age during year of birth and sex of puppy. 140 observations missing due to missing either serologic or molecular sire diagnostic status year of birth. 247 observations missing due to missing either serologic or molecular sire diagnostic status.
Table 4.
Multivariable Logistic Regression Analysis for Sire Risk Factors Associated with Leishmania Venereal Transmission with dam negative. Model C: Explanatory variables include sire’s qPCR and serology statuses and age during year of birth and sex of puppy. 1 28 missing observations due to missing either serologic or molecular sire diagnostic status.
Table 5.
Multivariable Logistic Regression Analysis for Risk Factors Associated with sire’s impact on Leishmania Venereal Transmission with dam positive. Model C: Explanatory variables include sire’s qPCR and serology statuses and age during year of birth and sex of puppy. 116 observations missing due to missing either serologic or molecular sire diagnostic status.
Fig 1.
Kaplan-Meier time to offspring diagnostic positive based on Sire’s Ever diagnostic status.
Proportion of uninfected refers to the proportion of dogs that were diagnostically negative via real-time PCR and serology. Solid blue represents the diagnostic status of pups from sire who were diagnostically negative via both qPCR and serology during their lifetime. Dashed red represents the diagnostic status of pups from sire who were diagnostically positive at any point during their lifetime via qPCR or serology. Shaded area represents variance around the mean. Number at risk displayed along x-axis. Log-rank test was used. (Chi-Square: 0.1984, p-value: 0.6560).
Fig 2.
Kaplan-Meier time to offspring diagnostic positive based on Sire’s qPCR diagnostic status during the year of birth.
Proportion of uninfected refers to the proportion of dogs that were diagnostically negative via qPCR. Solid blue represents the diagnostic status of pups from sires who were diagnostically negative before birth. Dashed red represents the diagnostic status of pups from sires who were diagnostically positive via qPCR before birth. Shaded area represents variance around the mean. 37 observations were excluded due to missing sire’s qPCR diagnostic status before birth. Number at risk displayed along x-axis. Log-rank test was used. (Chi-Square: 1.5437, p-value: 0.2141.
Fig 3.
Kaplan-Meier time to offspring diagnostic positive based on Sire’s serology status during the year of birth.
Proportion of uninfected refers to the proportion of dogs that were diagnostically negative via serology. Solid blue represents the diagnostic status of pups from sires who were diagnostically negative before birth. Dashed red represents the diagnostic status of pups from sires who were diagnostically positive via serology before birth. Shaded area represents variance around the mean. 41 observations were excluded due to missing sire’s serological status before birth. Number at risk displayed along x-axis. Log-rank test was used. (Chi-Square: 4.0301, p-value: 0.0447).