Table 1.
Yeast hemagglutination assays with human erythrocytes.
Fig 1.
Affinity chromatography with human erythrocyte stroma.
(A) Soluble components of the yeast-culture medium. (B) Yeast-homogenate from the H. capsulatum EH-53 isolate. AU = Adsorption Units at 280 nm. Arrowheads indicate the start of elution with 3% acetic acid.
Fig 2.
Intravascular hemagglutination mediated by H. capsulatum yeasts in a patient diagnosed postmortem with disseminated histoplasmosis and in a randomly captured infected bat.
Histopathological findings in the cecum and ileum sections revealed hemagglutination formed by clusters of erythrocytes containing yeasts (A-C, black arrows). Abnormal adhesion of erythrocytes to the vascular endothelium was observed (A and B, black arrowheads). The presence of schistocytosis was observed, based on the altered erythrocyte morphology (A-C, red arrowheads). In a liver histological section of a randomly captured H. capsulatum-infected bat, fungal yeasts adhering to erythrocytes were also observed (D, black arrows). Grocott-Gomori methenamine silver histochemistry. Bars: A-C = 25 µm; D = 5 µm.