Fig 1.
Schematic (not in scale) of SPR instrument set-up (a) with an exchangeable polymer chip with optical grating couplers, input DOCE and output DOCE (b), and the photograph of used chip (c).
Fig 2.
Representation of the arrangement of the layers.
1. polymer substrate with a 2. thin 50 nm gold layer (G, in yellow); 3. antibodies (Y, in gray); 4. Snake venom (spheres in red). The solutions were transported to the biochip by a peristaltic pump. Flow is essential due to the need to measure multiple analytes in a single assay. The flow rate varies between 5 and 10 Lmin−1, values directly related to the analyte, to optimize the adsorption and desorption process of the analyte on the gold film.
Fig 3.
Illustrative graph demonstrating the test stages that make up the results.
The blue markers on the graph indicate the time point at which the type of fluid in the pump reservoir was changed.
Fig 4.
Graphs of the moving average of the refractive indices of PBS, BAV, Crotalus Venom (CV) and Bothrops Venom (PB) as a function of time.
a) BAV 1:100, CV 1:7,500, PV 1:7,500; b) BAV 1:1,000, CV 1:7,500, PV 1:7,500. The blue markers on the graph indicate the time point at which the type of fluid in the pump reservoir was changed.
Fig 5.
Graphs of the moving average of the refractive indices of PBS, BAV, Crotalus Venom (CV) and Bothrops Venom (PB) as a function of time.
a) BAV 1:100, CV 1:60,000, PV 1:60,000; b) BAV 1:1,000, CV 1:60,000, PV 1:60,000. The blue markers on the graph indicate the time point at which the type of fluid in the pump reservoir was changed.
Fig 6.
Kinetics constant estimation for different concentration of BAV.
a) BAV 1:100, CV 1:7,500, PV 1:7,500; b) BAV 1:1,000, CV 1:7,500, PV 1:7,500; c) BAV 1:100, CV 1:60,000, PV 1:60,000; d) BAV 1:1,000, CV 1:60,000, PV 1:60,000.