Fig 1.
Small mammal collection site within Mata da Tapera Municipal Natural Park (MT), located in the Serra do Cipó district, Minas Gerais State, Brazil.
(A) General view of the MT area, showing its proximity to peridomestic environments. (B) Steel traps (Tomahawk model) baited with a mixture of fruit and cod liver oil emulsion or bacon, used for small mammal captures. Image provided by the authors and published under a CC BY license.
Fig 2.
Field xenodiagnoses of wild and synanthropic small mammals using laboratory-reared Lutzomyia longipalpis and Rhodnius neglectus in Mata da Tapera Municipal Natural Park (MT), Serra do Cipó district, Minas Gerais State, Brazil.
(A) Nectomys squamipes under anesthesia during xenodiagnoses with Lutzomyia longipalpis. (B) Fourth-instar nymphs of R. neglectus used in the procedure. Animals were maintained under sedation with xylazine (10 mg/kg) and ketamine (20 mg/kg) for approximately 30 minutes during xenodiagnostic exposure.
Table 1.
Small mammal species captured in Mata da Tapera Municipal Natural Park (Minas Gerais, Brazil), by collection month.
Fig 3.
Maximum Likelihood (ML) phylogeny of Leishmania spp. detected in small mammals based on 18S rRNA (V7-V8 region).
Node support is shown as SH-aLRT (%)/ ultrafast bootstrap (UFBoot, %) based on 1,000 replicates. The best-fit model selected by ModelFinder was HKY. The tree was rooted using Leptomonas pyrrhocoris as outgroup. Sequences generated in this study are highlighted in red.
Table 2.
Xenodiagnoses, parasite culture followed by 18S rRNA sequencing, and direct PCR followed by 18S sequencing results for small mammals captured in the Mata da Tapera Municipal Natural Park (Minas Gerais, Brazil) between 2023 and 2024. GenBank accession numbers are indicated in brackets.
Fig 4.
Maximum Likelihood (ML) phylogeny of Trypanosoma spp. detected in small mammals based on 18S rRNA (V7-V8 region).
Node support is shown as SH-aLRT (%)/ ultrafast bootstrap (UFBoot, %) based on 1,000 replicates. The best-fit model selected by ModelFinder was TN + F + G4. The tree was rooted with Leishmania braziliensis as outgroup. Sequences generated in this study are highlighted in red.