Table 1.
Crude prevalence of lymphatic filariasis in 16 villages of Belitung Timur district.
Fig 1.
Map of the study area in Belitung Timur District, Indonesia.
Belitung Island is divided into two districts: Belitung District (left) and Belitung Timur District (right). The study area, Belitung Timur District, includes sixteen labeled study villages. Among these, fourteen were classified as endemic (highlighted in pink) and two were non-endemic (in yellow). From the endemic villages, four high-endemicity villages were chosen for the Brugia Test Plus diagnostic assessment (red dots). Base map layer sourced from GADM for Indonesia (https://gadm.org/download_country.html, select “Indonesia”) and used under the GADM license (https://gadm.org/license.html). Map created using QGIS.
Fig 2.
The flow of study participants in Belitung Timur.
Table 2.
Percentage of Mf+ in each endemic village.
Fig 3.
Comparison of Fingerstick blood with and without anticoagulant on the same subject.
Row A and B show BT+ from blood samples without anticoagulant in duplicates while row C shows BT+ from EDTA-treated blood.
Fig 4.
Appearance of the test line when run with fresh blood, without anticoagulants, compared to Mf density.
Table 3.
Percentage of anti-filarial IgG4 positivity in microfilaremic patients based on gender.
Fig 5.
Comparison of 3 matrices from the same patient with EDTA whole blood (A), EDTA plasma (B), and DBS (C).
Whole blood and DBS give very tenuous test lines, while plasma gives a more easily visible test line.
Table 4.
Percentage of IgG4 positivity in Mf negative individuals.
Table 5.
Brugia Test Plus results from seven elementary schools.
Table 6.
Pairwise inter-reader agreement in BT+ interpretation.