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Fig 1.

The growth of pig (a) and guinea pig (b) derived B. coli trophozoites in DMEM medium.

Observation of binary fission (c) and conjugation (d) in pig-derived B. coli trophozoites, but only binary fission in guinea pig-derived isolates during growth (e).

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Fig 2.

Homology analysis of complete amino acid sequence of β-tubulin.

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Fig 3.

β-tubulin protein domains of different species origin.

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Table 1.

Location information of β-tubulin sequences in the draft whole genomes.

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Fig 4.

Phylogenetic analysis based on full-length β-tubulin sequences.

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Fig 5.

Comparison of PCR amplification results for β-tubulin and ITS loci (M: Marker; N: Negative control).

Lanes 1-8 show the ITS locus, and lanes 9-16 show the β-tubulin locus. Eight samples were selected for analysis, in the following order from left to right: pig (n = 3), sheep (n = 2), cattle (n = 1), and guinea pig (n = 2).

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Fig 6.

Phylogenetic tree constructed based on β-tubulin sequences.

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Fig 7.

Phylogenetic tree constructed based on ITS sequences.

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