Fig 1.
CONSORT diagram for dam and puppy study inclusion.
Table 1.
Weighted factors calculated based on year of birth distributions of sample and entire puppy population.
Table 2.
Demographics of animals enrolled in the study. Vaccination regime was considered complete with 3 doses of LeishTec.
Fig 2.
Evaluation of mortality, clinical signs and canine leishmaniosis diagnosis in puppies born to infected and placebo-vaccinated or infected and vaccinated dams.
Dogs born to placebo-vaccinated or vaccinated dams were counted and graphed based on mortality (A), manifestation of the number of CanL associated clinical signs (B, C), CanL diagnosis by litter, assessed by kqPCR, DPP or ELISA (D) and loss of negative diagnosis for CanL (E) Kaplan-Meier curve for dogs born to vaccinated (blue line) and unvaccinated (red line) infected dams. Graphs and analysis were performed in Graph Prism (version 8) and R (version 3.6.2).
Fig 3.
Evaluation of adaptive response and Leishvet clinical in puppies born to infected and placebo-vaccinated or infected and vaccinated dams.
Adaptive responses measured by ratio of CD4 IFN-g by CD4 IL-10 secreting cells was done, comparing dogs born to placebo-vaccinated or vaccinated dams (A). Clinical manifestation, based on LeishVet system, of the number of CanL, was also performed on both cohorts. Data was acquired every 3 months from 2019 – 2021. Graphs and analysis were performed in Graph Prism (version 8) using 2way ANOVA. Dogs born to vaccinated mothers: 5, dogs born to unvaccinated mothers: 3.