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Fig 1.

Location of the study site in Tekik de Regil, Yucatán, México, and distribution (sketch) of the study arms: Control (PPC and NPC), red spots are premises positive, and the white spots are premises negative for Triatoma dimidiata infestations.

The spots with crosses receive treatment (PPT and NPT). The base map was created in QGIS 3.36.1 Maidenhead (qgis.org), using layers OpenStreetMap (http://www.openstreetmap.org/), under the Open Database License (https://www.openstreetmap.org/copyright).

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Fig 2.

Examples of the main peridomestic ecotopes where T. dimidiata was found and collected at the study site.

A) Doghouse, B) Concrete blocks, C) Piled rocks D) Chicken coop made of concrete blocks, E) Example of a sketch of premises with their peridomestic ecotopes, F) Piled construction material, G) traditional chicken coop made with wire mesh, rocks, and wooden pillars, H) Rabbit hutch using concrete blocks, I) piled firewood in the backyard. Photo by Arisqueta-Chablé.

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Fig 3.

Residual spraying applied inside and outside the houses, in positive/potential ecotopes of triatomines.

Photo by Arisqueta-Chablé.

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Table 1.

Summary of premises infested by Triatoma dimidiata collections by method (TMC = Timed Manual Collections, HC = Householder Collections) and site at baseline survey (pre-intervention- March 2022). Infestation refers to premises positive to triatomine.

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Table 2.

Summary of Triatoma dimidiata abundance by life stage, sex, and site collections at baseline survey (pre-intervention- March 2022).

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Table 2 Expand

Table 3.

Summary of peridomestic ecotopes infested by Triatoma dimidiata at baseline survey (pre-intervention, March 2022).

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Fig 4.

(A) Proportion of peridomiciles detected infested by Triatoma dimidiata at baseline found positive during the 6-month follow-up post-spraying. (B) Cumulative number of peridomiciles was found positive by T. dimidiata.

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Table 4.

Infestations of peridomestic ecotopes by Triatoma dimidiata post-intervention (May-October 2022) in the control and treatment arms. The type and number of positive ecotopes (surveyed), percentage of infestation, percentage of triatomine bugs and P-value of the Chi-square contingency table analysis are listed. signif. codes: *P < 0.05, **P < 0.10.

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Table 5.

Random-effects logistic regression model (GLMM) of Triatoma dimidiata infestation odds 6 months after Actellic 300CS residual spraying. S.E. standard error, signif. codes: * P < 0.05, **P < 0.10.

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