Fig 1.
Colony Morphology of B. rochalimae on blood agar plates.
Table 1.
Data on B. rochalimae strains and clinical, demographic, and diagnostic information of patients in which it was identified.
Fig 2.
Heatmap of ortho-ANI distances, including patient-isolated strains of this study (Br-94-INS; Br-131-INS; Br-132-INS; Br-136-INS; Br-174-INS).
Fig 3.
SNP-based RAxML Maximum Likelihood (ML) phylogenetic tree of B. rochalimae with bootstrap node support.
The tree includes strains isolated from patients (Br-94-INS; Br-131-INS; Br-132-INS; Br-136-INS; Br-174-INS) and depicts strains and clades associated with B. rochalimae. It features a distant strain (A), represented by a red dotted branch (not to scale), and four clades: B (olive branches), C (green branches), D (light blue branches), and E (pink branches). To improve visualization, the branch length of strain A was compressed. The ATCC BAA-1498 strain is indicated by a black diamond.
Fig 4.
Comparison of functional annotations in coding sequences (CDS) obtained by GO, compared across clades (left) and strains (right), including patient-isolated strains of this study (Br-94-INS; Br-131-INS; Br-132-INS; Br-136-INS; Br-174-INS). Each cell reflects the number of unique CDS found in a particular strain or clade.
Fig 5.
Comparison of subcellular localization in coding sequences (CDSs) predicted by BUSCA across clades (Bottom) and strains (Top), including patient-isolated strains from this study (Br-94-INS; Br-131-INS; Br-132-INS; Br-136-INS; Br-174-INS). Each cell indicates the number of unique CDSs identified in a particular strain or clade.
Table 2.
Differential genes of B. rochalimae.
Fig 6.
Comparison of virulence factors in coding sequences (CDSs) identified using VFDB across across strains (bottom) and clades (top), including patient-isolated strains from this study (Br-94-INS, Br-131-INS, Br-132-INS, Br-136-INS, and Br-174-INS). Each cell represents the number of unique CDSs found in a particular strain or clade.
Fig 7.
Principal Component Analysis based on GO results, show per strain.
Arrow lengths are proportional to category contribution on both axes.
Fig 8.
Total evidence Maximum Likelihood phylogenetic tree constructed using a partial matrix of seven regions (16S, 16S-23S, ftsZ, gltA, groEL, rpoB and ssrA).
Bootstrapsupports values ≥ 50 are indicated above major branches. The tree distinguishes between Rodent clade (blue circles) and non-rondent clade (red circles). The presence or absence of each region is indicated by yellow and light blue squares. Genomes generated in this study are highlighted with triangles, while genomes downloaded from NCBI are marked with stars. Tip labels include the strain name, host, and country of origin.