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Table 1.

B-cell epitope prediction.

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Fig 1.

SDS-PAGE (12%) analysis of the fusion protein expressed in prokaryotes.

M, the protein molecular weight marker. Lane 1, the purified fusion protein.

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Table 2.

Comparison of serum OD450 results in different groups.

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Table 3.

Positive predictive value and negative predictive value at different cut-off values.

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Fig 2.

I-ELISA detection of human serum samples.

A, the ROC curve for the human serum samples, illustrating the diagnostic sensitivity and specificity of the fusion protein-based assay. B, a dot plot representation of the OD450 values for both positive and negative human serum samples, with each dot representing an individual sample.

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