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Fig 1.

Study Area.

Map of the location of the three Merida suburbs (inset): (A) San Lorenzo, (B) Itzincab, (C) ACIM. Clusters of control (red blocks), MD-CD (green blocks), and CD-MD (blue blocks) arms are shown. The base map was created in QGIS 3.36.1-Maidenhead (qgis.org) using layers OpenStreetMap (http://www.openstreetmap.org/), under the Open Database License (https://www.openstreetmap.org/copyright).

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Fig 2.

Entomological impact on the abundance of Ae. aegypti.

(A) Mean number (error bars indicate 95% CI) of Ae. aegypti adults per house and per treatment for each entomological survey before and after the switch of treatment arms (indicated as a vertical dashed line). (B) Mean number of blood-fed Ae. aegypti females per house per survey date and treatment. Note that during the last 8 weeks of the trial, mosquito abundance was very low.

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Table 1.

Model selection.

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Table 2.

Results from the optimal GLM model.

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Table 3.

GLMM model comparisons of treatment and control arms: impact of metofluthrin emanators vs number of Ae aegypti per house.

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Fig 3.

Estimated number of Ae. aegypti from the best fitting GLMM.

Data presented as predicted means ± 95% CI. Different letters indicate statistically significant differences (P < 0.05).

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Table 4.

Predicted Incidence Risk Ratio (IRR) for attempted landings. Negative-binomial GLMM results comparing the mosquito landings, between the treatment arm (CD and MD) and the control. The efficacy of intervention (%Efficacy = 1-IRR*100) is also shown.

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Fig 4.

Predicted number of attempted landings by study arm.

Predictions were obtained from a GLMM with a Poisson link function. The vertical black line shows the separation of surveys before and after the switch in study arms.

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Fig 5.

Comparison of Resistant Allele Frequencies: Resistant homozygous forms (RR) are represented as LL, II, and CC, respectively, at the top of the graph, while sensitive homozygous forms are denoted as VV, VV, and FF.

(A) Displays a comparison of resistant allele frequencies across the study arms. (B) Shows all arms combined, comparing resistant allele frequencies between the baseline and the 4th replacement cycle.

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