Fig 1.
Overview of the location and the seasonality of cholera cases in the study area.
(A) Map of the DRC with population density (log transformed), boundaries of health zones in white, major roads in light green, a red box around the health zones of Kalemie and Nyemba. (B) Detail of red box from A, red circle on Kalemie city, Lake Tanganyika in blue. Low population density in grey, high in red. Health zones of Kalemie and Nyemba outlined in dark red. (C) Weekly number of reported suspected cholera cases (based on the International Organization for Standardization (ISO) system) in the health zones of Kalemie and Nyemba from 2002 to 2014 [25], typical rainy season weeks shaded in grey. Direct links to the map layers are: https://www.gadm.org/download_country.html, https://www.globio.info/global-patterns-of-current-and-future-road-infrastructure, https://landscan.ornl.gov, https://wbwaterdata.org/dataset/africa-water-bodies-2015.
Table 1.
Interpretation on the model parameters.
Fig 2.
Incident cases, model fit, and variation of the percentage of infected, recovered, and susceptible over time.
(A) Weekly reported suspected cholera cases residing in the city of Kalemie (empty circles) from November 2013 to February 2016 and mean model prediction of the reported weekly cholera cases (dark line) and its 95% credible interval (grey envelope) (B) Mean model estimates of the percent age of the population infected (prevalence), recovered, and susceptible (dark lines) and their 95% credible interval (grey envelopes) from November 2013 to February 2016. Typical rainy seasons are shaded in grey, the timing of the distribution of vaccine doses in vertical dashed grey lines, and the incremental implementation of the improvements in water and sanitation is indicated by the widening and darkening triangle between A and B.
Fig 3.
Estimated impact of the components of the intervention and impact of alternative vaccination strategies.
(A) Mean model predictions of the percentage of infected, recovered, and susceptible in the population considering: the intervention as it happened of WASH and vaccination (dark blue), WASH only (blue), vaccination only (green), and no intervention of either WASH or vaccination (yellow) from November 2013 to February 2016. The incremental implementation of the improvements in water and sanitation is indicated by the widening and darkening triangle in the top panel. Typical rainy seasons are shaded in grey and the timing of the distribution of vaccine doses is shown in dashed grey lines. (B) Violin plots of numbers of additional cholera cases at the end of the study period with WASH only (blue), vaccination only (green), or no intervention (no WASH and no vaccination) (yellow) compared to the intervention as it happened for WASH and vaccination. The error bars, the filled circles, and the horizontal bars indicate the 95% credible interval, the medians, and the means respectively. (C) Heatmap of the mean number of cases avoided by changing the timing and the coverage of a vaccination campaign compared to a scenario without intervention. The timing of the distribution of vaccine doses for the intervention as it happened is indicated by dashed grey lines.
Fig 4.
Contributions of the environmental reservoir in cholera transmission.
(A) Mean model predictions of the percentage of infected, recovered, and susceptible individuals in the population with environmental contamination and exposure (EC+EE) (light blue), environmental contamination only (no environmental exposure) (EC) (beige), and environmental exposure only (no environmental contamination) (EE) (grey) from November 2013 to February 2016. Typical rainy seasons are shaded in grey. (B) Violin plots of numbers of cholera cases avoided by the end of the study period with only EC (beige), or only EE (grey) compared to a scenario with EC and EE. The error bars, the filled circles, and the horizontal bars indicate the 95% credible interval, the medians, and the means respectively. All the scenarios considered in A and B assume that no intervention occurred. (C) Mean prediction of the variation of the environmental (light orange line) and interhuman (light green line) components of the force of infection and their 95% credible interval (light orange and light green envelopes) from November 2013 to February 2016. The light orange and light green dashed lines indicate the mean values of the environmental exposure rate (βe) and interhuman transmission rate (βh), respectively. (D) Left: Mean prediction of the variation of the environmental net bacterial growth (φt−ε) (dark line) and its 95% credible interval (grey envelope) from November 2013 to February 2016. Right: Violin plot and boxplot of the distribution of the mean prediction of the net bacterial growth rate from November 2013 to February 2016. The dashed black line indicates 0: values below 0 show net decay and values above 0 show net growth.