Fig 1.
Screening and diagnostic workflow highlighting ZIKV detection among DENV-positive and DENV-negative cases.
The "Not Done" category represents enrolled cases whose RT-PCR was not conducted.
Table 1.
Clinico-demographic features of ZIKV positive cases (n = 5) at the time of enrolment.
Fig 2.
Technical performance of the probe-based real time RT-PCR.
A) Amplification curves of four cases with ZIKV mono-infection have been depicted with corresponding positive control of ZIKV, each containing different viral loads with corresponding threshold cycle (Ct) values; B) amplification curves of Case-4 with co-infection have been depicted by two different colors with corresponding positive controls of DENV and ZIKV.
Fig 3.
The maximum likelihood phylogenetic tree of 89 E gene coding sequences of ZIKV circulating worldwide.
The tree was constructed using the bestfit substitution model with an ultrafast bootstrap of 1000 replicates. The Bangladeshi (BD) strains are denoted by purple asteroids. All three BD ZIKV strains were closely related to each other and classified under the Asian sublineage. They formed a distinct node, grouping together with Cambodian and Chinese strains from 2019.