Fig 1.
Procedure for isolation of DNA from stool samples.
After sample collection, isolation of DNA occurred in three steps. (A) Samples were washed, to remove the ethanol used for storage and preservation. (B) Samples were scooped into OMNI 96-well 1.4 mm ceramic bead-beating plates. (C) Following overnight incubation, DNA extraction was performed.
Table 1.
Primers and probes used in DeWorm3 assays.
Table 2.
Infection characteristics for spiked stool standards.
Table 3.
Characteristics of contrived standards used for limit of detection testing.
Fig 2.
Assessment of sample heterogeneity.
Illustration depicting how each sample used in the assessment of sample heterogeneity generated eight points of data. Blue fills indicate samples subjected to reference protocols (manual extraction and singleplex qPCR) and green fills indicate samples subjected to DeWorm3 protocols (semi-automated extraction and multiplex qPCR).
Fig 3.
Results of control validation.
(A) Box plot depicting the range of CT values for A. suum detection. (B) Box plots depicting CT values for all B. atrophaeus results obtained when running both DeWorm3 assays on a panel of STH-negative, B. atrophaeus-positive samples.
Table 4.
DeWorm3 assay accuracy at the individual extraction and technical replicate levels.
Table 5.
By-species results of limit of detection testing.