Fig 1.
In the main map, red dots are placed within 2km of the location of each recruited farm (points are jittered to preserve anonymity). SNP: Serengeti National Park; GGR: Grumeti Game Reserve; IGR: Ikorongo Game Reserve. On the top left corner, a single red dot indicates the location of the study area in relation to the whole of Tanzania. Dark green on the map denotes wildlife protected areas [41], whereas light green are non-wildlife protected areas [42]. Most households in this area are livestock keepers and they depend on livestock including cattle, sheep and goats for their income [43]. According to the cattle census for branding [44], the district had 385,542 cattle in 2018. Like other areas in the Northern Tanzania, especially those near national parks and game reserves where tsetse vectors and wildlife hosts are present [45], livestock owners living close to the protected area boundaries are at high risk of AAT, and it is a concern to the members of the community [14].
Fig 2.
Decision tree designed to interpret laboratory results and determine trypanocide administration outcomes.
TX: trypanocide administration day; FU1-4: follow up one-four (one week, one month, two months and three months post treatment). ISM can be use for both treatment and prophylaxis, so both outcomes are considered. DA and HM are used for treatment only.
Fig 3.
Proportion of cases that showed at least one clinical sign consistent with AAT on veterinary examination, and proportion that tested PCR positive (i.e. diagnosis) for at least one of T. brucei, T. congolense or T. vivax at the time of treatment, split between treatment events where farmers indicated they were administering prophylaxis compared to treating animals with AAT *Clinical signs consistent with diarrhoea, emaciation, rough hair coat, pale mucous membranes, PCV<25 or BCS < = 1.5 were classified as AAT. If clinical signs were observed but they were not consistent with AAT, these events were classed as ‘other’. If no clinical signs were observed, animals were classed as ‘healthy’.
Fig 4.
Proportion of adequate trypanocide administrations out of 268 observed administration events.
Adequacy was established based on four criteria, namely trypanocide dose, route of administration, competency of administration and drug storage conditions.
Fig 5.
Proportion of adequate doses, overdoses and underdoses for each trypanocide administered in this study.
ISM = isometamidium chloride; DA = diminazene aceturate; HM = homidium chloride. For ISM, dose categories are based on recommendations to achieve prophylaxis protection (0.5–1 mg/kg). Where multiple trypanocides were combined in one administration, each drug is included separately. The p-value of a Fisher’s exact test to compare the association between trypanocide and dose type is reported at p<0.001.
Fig 6.
Number of treatment and prophylaxis outcomes grouped by trypanocide and coloured according to the adequacy of administration.
Treatment outcomes for cattle not infected with Trypanosoma at the time of treatment are not reported. ISM = isometamidium choride; DA = diminazene aceturate; HM = homidium chloride.
Table 1.
Summary of the values obtained from the regression model evaluating risk factors for prophylaxis failure following administration of isometamidium chloride.
Fig 7.
Ratio between measured and expected drug concentrations in the trypanocide samples analysed by HPLC.
Red shapes indicate samples of diminazene aceturate (DA), while blue shapes represent samples of isometamidium chloride (ISM). Triangles denote samples sourced from the same batches used in trypanocide administrations observed in this study whist circles indicate samples purchased from drug shops and markets. Anonymized labels on the X-axis correspond to different trypanocide brands. The green dashed line represents a perfect match between measured and expected drug concentrations, while the orange dashed lines indicate a deviation of ±15% from the ideal ratio of 1.