Fig 1.
One-step amplicon-based NGS in this study.
A. Schematic diagram of HTNV genome covered by amplicons produced by 10 pairs of primers. B. Amplicons obtained using the primers were validated through electrophoresis. C. The workflow of one-step amplicon-based NGS.
Fig 2.
“*”: Among these 32 patients, virus isolation was performed simultaneously using plasma (isolate n = 0) and PBMCs (isolates n = 3) from 7 paitens, and virus isolation was performed using only PBMC from 25 patients (isolates n = 10).
Fig 3.
The IFA images of HTNV-infected Vero-E6 cell and Vero-E6 cells control.
HTNV-infected Vero-E6 cells show specific bright green fluorescent dots indicating the virus was isolated successfully (A), while the control Vero-E6 cells show no bright green fluorescent dots (B).
Fig 4.
TEM images of HTNV particles (isolate JXGAHu98/2021) in Vero-E6 Cell.
A vesicle of the HTNV-infected cell was magnified, virions were observed inside the vesicles, and arrows pointed to the HTNV particles. “N”: the nuclear of the cell.
Table 1.
Summary genome sequencing of HTNVs from patients using one-step amplicon-based NGS.
Fig 5.
Phylogenetic tree of the orthohantavirus using the complete M sequence.
The scale bars indicate the number of nucleotide substitutions per site. Strains labeled in red represent HTNVs from 14 patients in this study and those in blue represent HTNV isolates from rodents in Jiangxi but not in this study. PUUV, Orthohantavirus puumalaense. SEOV: Orthohantavirus seoulense.
Table 2.
Characteristics of the 53 patients with HFRS identified by RT-qPCR, Jiangxi, 2020–2022.
Fig 6.
The proportion of patients with elevated liver and renal markers (n = 53).
AST, LDH, ALT, ALP, GGT, and DB were liver function indicators, and proteinuria, BUN, and SCR were renal function indicators. The ULN levels of AST, LDH, ALT, ALP, GGT, DB, BUN, SCR, and proteinuria are 40 U/L, 240U/L, 50 U/L, 135 U/L, 60 U/L, 6 μmol/L, 120 μmol/L, 7.1 mmol/L, and 0.15 g/L, respectively.