Fig 1.
1The plasma sample was taken after birth in the clinical follow-up. In two pairs, informed consent was not found.
Table 1.
Clinical characteristics of the study cohort mother and child pairs.
Fig 2.
Placentally-transferred IgG-NS1.
A and B. Correlation analysis of the relative plasma levels of ZIKV (A) and DENV1-4 (B) IgM-NS1 between mothers and children. C and D. Correlation analysis of the relative levels of ZIKV (C) and DENV1-4 (D) IgG-NS1 between mothers and children. E and F. Correlation and non-linear regression analysis of the relative levels of ZIKV (E) and DENV1-4 (F) IgG-NS1 in relation to the children age. In all the cases, the rho and p-value of the Spearman test are shown. The dashed lines indicate the ELISA limit of detection.
Fig 3.
Poor association between IgM-NS1 antibodies and CZS-related microcephaly.
A and C. Frequency of ZIKV and DENV1-4 IgM-NS1 positivity rate in mothers (A) and children (C) with or without microcephaly; Fisher’s test. B and D. Relative levels of ZIKV and DENV1-4 IgM-NS1 in mothers (B) and children (D) with or without microcephaly; Mann-Whitney test. The dashed lines indicate the ELISA limit of detection. NS: Not statistically significant. *p < 0.05.
Fig 4.
Association between IgG-NS1 antibodies and CZS-related microcephaly.
A. Frequency of DENV1-4 IgG-NS1 positivity rate in mothers of children with or without microcephaly. B. Relative plasma levels of ZIKV and DENV1-4 IgG-NS1 in mothers of children with or without microcephaly. C and D. DENV1-4/ZIKV IgG-NS1 ratio in mothers of children with or without microcephaly (C), and the respective ROC curve analysis (D). E. Frequency of ZIKV and DENV1-4 IgG-NS1 positivity rate in children with or without microcephaly. F. Relative plasma levels of ZIKV and DENV1-4 IgG-NS1 in children with or without microcephaly. G and H. DENV1-4/ZIKV IgG-NS1 ratio in children with or without microcephaly (G), and the respective ROC curve analysis (H). In A and E, the p-value of the Fisher’s test is shown. In B, C, F, and G, the p-value of the Mann-Whitney test is shown. The dashed lines indicate the ELISA limit of detection. NS: Not statistically significant. **p < 0.01; ***p < 0.001.