Table 1.
Primer sequences and annealing temperatures of Plasmodium genus and species-specific PCR assays.
Fig 1.
Limit of detection workflow for (A) P. genus, (B) Plasmodium species-specific, and (C) P. genus dried blood spot PCR assays.
Abbreviations: DBS = dried blood spot; LOD = limit of detection; qPCR = real-time quantitative PCR; Pk = P. knowlesi; Pf = P. falciparum, Pv = P. vivax; Pcyn = P. cynomolgi; RT = reverse transcription.
Fig 2.
The median LOD and fold-change with and without reverse transcription for PCR assays to detect Plasmodium species.
(A) The median LOD (parasites/μL) for P. genus assays using P. knowlesi, P. vivax, and P. cynomolgi samples without reverse transcription; (B) The median LOD (parasites/μL) for assays with reverse transcription. Error bars represent the IQR. Abbreviations: P. genus, Plasmodium genus; Pk, P. knowlesi; Pv, P. vivax; Pc, P. cynomolgi.
Table 2.
Limit of detection (LOD) of Plasmodium genus and species-specific PCR assays.
Table 3.
Limit of detection of the 18S rRNA P. genus qPCR assay for P. knowlesi dried blood spot samples.