Fig 1.
(a) M. tuberculosis colonies as seen under the inverted microscope (x 400). (b) Characteristic serpentine cords of M. a tuberculosis observed under the inverted microscope (x 400).
Table 1.
Demographic characteristics and patient profile.
Fig 2.
Flowchart of patient enrolment and analysis of results by different detection methods.
Table 2.
Sensitivity, specificity, positive predictive value and negative predictive value of DST by modified MODS for the detection of M. tuberculosis and INH or RIF resistance.
Fig 3.
GenoType MTBDR-plus strip showing the mutational pattern of representative isolates (Lane 1) Multidrug-resistant tuberculosis, rpoB S531L mutation.
(Lane 2) Multidrug-resistant tuberculosis, rpoB H526R mutation. (Lane 3) Multidrug-resistant tuberculosis, katG S315T2 mutation. (Lane 4) M. tuberculosis, INH monoresistant (katG S315T1 mutation). (Lane 5) M. tuberculosis, INH heteroresistant (mutation in katG 315 region). (Lane 6) Multidrug-resistant tuberculosis, InhA T8C mutation. (Lane 7) M. tuberculosis, INH heteroresistant (mutation in InhA -15 region). (Lane 8) M. tuberculosis, INH heteroresistant (mutation in InhA -16 region). (Lane 9) Mycobacterium tuberculosis, susceptible to isoniazid and rifampicin. (Lane 10) H37Rv Positive control. (Lane 11) Negative control (distilled water).
Table 3.
The mutational pattern of drug-resistant Mycobacterium Tuberculosis strains.
Fig 4.
Time to positive culture for modified MODS, Lowenstein–Jensen (LJ) and APM.
Fig 5.
Time to LJ and modified MODS positivity in relation to smear grade.