Table 1.
The primers and controls used for the PCR detection of cfDNA in the sera collected from 35 sheep with cystic echinococcosis.
Table 2.
Materials used in DNA extraction from 0.5 and 2ml serum samples.
Fig 1.
The location of the amplified gene fragments and the primers (nad F1, nad R2, and nad R1) in the PCR and nested PCR on “Echinococcus granulosus mitochondrion, complete genome, NCBI Reference Sequence: NC_044548.1”. The numbers indicate the beginning and the end of the fragment in the genome.
Table 3.
Detection of cfDNA using cox1 primer from different volumes of sheep serum using standard and semi nested PCRs.
Table 4.
Detection of cfDNA in different volumes of sheep sera using standard and semi nested PCRs and nad1 primer.
Fig 2.
Agarose gel electrophoresis of the standard PCR and semi-nested PCR products from sheep serum samples in 500 and 2000 ml volums. Lane M. 100 bp DNA molecular size marker, lanes 1 and 7. standard PCR in the 0.5 ml volume, lanes 2 and 8. semi-nested PCR in the 0.5 ml volume, lanes 3 and 9 are standard PCR in the 2 ml volume and the 5 μl template DNA, lanes 4 and 10 are semi-nested PCR in the 2 ml volume and the 5 μl template DNA, lane 5 and 11 are standard PCR in the 2 ml volume and the 10 μl template DNA, lanes 6 and 12 are semi-nested PCR in the 2 ml volume and the 10 μl template DNA, lane13 is a representative negative clinical samples, lanes 14 and 15. negative and positive controls, respectively.