Fig 1.
Release areas within Bello, Medellín and Itagüí.
Dark blue, light blue and yellow shading denote Bello, Medellín and Itagüí respectively (map produced in QGIS version 3.28.3 using administrative boundaries for the municipal governments of Bello (https://www.datos.gov.co/Ordenamiento-Territorial/Divisi-n-Pol-tico-Administrativa-Barrios-Bello-Ant/pnhh-ccwd), Medellín (https://data.metabolismofcities.org/library/maps/35283/view/), and Itagüí (https://www.datos.gov.co/Ordenamiento-Territorial/Localizaci-n-Geogr-fica-de-los-Barrios-del-Municip/didi-drqa)). The initial París release area is coloured orange with the insert showing a close up of the area. The striped pattern within the insert indicates the initial trial.
Table 1.
Public acceptance of implementing the Wolbachia method within Bello, Medellín and Itagüí.
Table 2.
wMel-infected Aedes aegypti lines for release in Bello, Medellín and Itagüí.
Fig 2.
Wolbachia establishment in París comuna, Colombia.
The lines (left axis) represent the percent of Ae. aegypti screened that were infected with wMel Wolbachia in the initial release area in the París neighbourhood, shown with an orange line, and the wider París comuna excluding the initial release area, shown with a yellow line. Yellow shading indicates release periods in the París neighbourhood. Green shading indicates release periods in the wider París comuna. The stacked bars (right axis) indicate the number of Ae. aegypti screened within the París neighbourhood (light blue) and in the wider París comuna (dark blue). Monitoring events with less than five screened mosquitoes were omitted.
Fig 3.
Wolbachia infection prevalence over time in Aedes aegypti mosquitoes in ten deployment areas of Bello, Colombia.
The orange line (left axis) represents the percentage of Ae. aegypti tested that were infected with wMel Wolbachia. Phase 1 releases, using the wMel-COL line are shown with yellow shading. Phase 2 releases, using the wMel-COL2 line, are shown with green shading. The blue bars (right axis) indicate the number of Ae. aegypti tested. Months with fewer than five Ae. aegypti tested have been omitted (n = 3 in Guasimalito; n = 2 in Santa Ana; n = 1 in La Cumbre).
Fig 4.
Wolbachia infection prevalence over time in Aedes aegypti mosquitoes in 12 deployment areas of Medellín, Colombia.
The orange line (left axis) represents the percentage of Ae. aegypti tested that were infected with wMel Wolbachia. Phase 1 releases using the wMel-COL line are shown with yellow shading. Phase 2 releases, using the wMel-COL2 line release periods are shown with orange and green shading. The blue bars (right axis) indicate the number of Ae. aegypti tested. To aid visualisation, months with greater than 1000 Ae. aegypti tested were capped at 1000 (n = 3 in Guayabal; n = 1 in La Candelaria).
Fig 5.
Wolbachia introgression in the Medellín case control study, in the Aburrá Valley, Colombia.
The orange line (left axis) represents the percent of Ae. aegypti screened that were infected with wMel Wolbachia. Phase one releases, using the wMel-COL line, are shown with yellow shading. Phase two releases, using the wMel-COL2 line are shown with green shading. The blue bars (right axis) indicate the number of Ae. aegypti screened. Months with fewer than five Ae. aegypti tested have been omitted (n = 1 in Popular; n = 1 in Santa Cruz).
Table 3.
Wolbachia establishment in Bello & Medellín by comuna.
Fig 6.
Wolbachia introgression in Itagüí, Colombia.
The orange line (left axis) represents the percent of Ae. aegypti screened that were infected with wMel Wolbachia. Green shading indicates release periods using the wMel-COL2 line. The blue bars (right axis) indicate the number of Ae. aegypti screened. Monitoring events with less than five screened mosquitoes are omitted.