Table 1.
Classification of groups for differential protein expression analysis using ANOVA of Ae. aegypti field strain from the dengue hotspot area and laboratory strain.
Table 2.
Primer sequences of the selected and reference genes.
Table 3.
Knockdown time (min) KT50 and KT99 against 0.75% permethrin and 31.25mg/l temephos of Ae. aegypti female adults and larval from field and laboratory strains.
Table 4.
Insecticide susceptibility status of Ae. aegypti female adults and larvae against 0.75% permethrin and 31.25mg/l temephos.
Fig 1.
Histograms present three replicates of Ae. aegypti identified proteins.
A. Adults. B. Larvae. Note: FAR/FLR: Adult/Larvae Ae. aegypti field permethrin resistant strain, FAN/FLN: Adult/Larvae Ae. aegypti field strain that was not exposed to permethrin, SAE/SLE: Adult/Larvae Ae. aegypti laboratory strain that was exposed to permethrin. SAN/FLN: Adult/Larvae Ae. aegypti laboratory strain that was not exposed to permethrin.
Fig 2.
Multi-scatter plot and Pearson correlation coefficient of Ae. aegypti identified proteins.
A. Adults B. Larvae.
Fig 3.
Hierarchical clustering of the identified proteins in adults and larvae Ae. aegypti.
Red black, and green bars represent high: normal, and low protein expressions. Note: FAR; Field strain adult Ae. aegxpti permethrin resistant. FAN: Field strain adult Ae. aegxpti not exposed to permethrin. SAE: Laboratory strain adult Ae. aegxpti exposed to permethrin. SAN: Laboratory strain adult Ae. aegxpti not exposed to permethrin. FLR: Field strain Ae. aegxpti larvae temephos resistant. FLN: Field strain Ae. aegxpti larvae not exposed to temephos. SLE: Laboratory strain Ae. aegxpti larvae exposed to temephos. SLN: Laboratory strain Ae. aegxpti larvae not exposed to temephos. The numbers (1,2,3)that follow the initial three letters indicate the replicate.
Fig 4.
PCA of Ae. aegypti identified proteins.
A. Adults B. Larvae.
Fig 5.
Volcano plots show differentially expressed proteins in adults Ae. aegypti.
A. Field permethrin-resistant strain versus laboratory strain that was exposed to permethrin B. Field strain versus laboratory strain that were not exposed to permethrin. Notes: FAR: Field adult permethrin resistant strain SAE: Adult laboratory strain that was exposed to permethrin FAN: Field adult strain that was not exposed to permethrin. SAN: Laboratory adult strain that was not exposed to permethrin.
Table 5.
Example of the up-regulated and down-regulated proteins in adult Ae. aegypti.
Fig 6.
Volcano plots show differentially expressed proteins in larvae Ae. aegypti.
A. Field temephos-resistant strain versus laboratory strain that was exposed to temephos B. Field strain versus laboratory strain that were not exposed to temephos. Notes: FLR: Field larvae temephos resistant strain SLE: Laboratory larvae strain that was exposed to temephos FLN: Field larvae strain that was not exposed to temephos SLN: Laboratory larvae strain that was not exposed to temephos.
Table 6.
Example of the up-regulated and down-regulated proteins in larvae Ae. aegypti.
Table 7.
Example of significant functional enrichment proteins in adult and larvae Ae. aegypti.
Table 8.
Gene expression fold change (FC) of Ae. aegypti.
Fig 7.
Differentially expressed proteins PPI in permethrin-resistant Ae. aegypti.
Fig 8.
Differentially expressed proteins PPI in temephos-resistant Ae. aegypti.
Table 9.
Functional ontology enrichment of differentially expressed proteins in adult Ae. aegypti permethrin-resistant strain.