Table 1.
Treatments, routes, doses and frequency.
Fig 1.
Image created with Biorender.
Table 2.
Treatment groups.
Fig 2.
Body weight, liver weight, and liver weight/body weight ratio of mice at 90 dpi, 120dpi and 142dpi.
Body (A) and liver (B) weights were taken at time of euthanasia. A ratio was calculated to normalize results. *P <0.05; ***P <0.0005; ****P <0.0001.
Fig 3.
Parasite burden and amount of inflammatory infiltrate in the liver.
T. cruzi parasite burden expressed as parasite equivalents per mg of tissue (A) was determined using qPCR and inflammatory infiltrate was determined via histopathology analysis using ImageJ (B). *P <0.05; **P <0.005. $ $ $ P<0.001 when compared to Infected Untreated at 90dpi.
Fig 4.
Impact of therapeutic interventions on the inflammatory infiltrate of liver tissue in mice.
Representative H&E stained sections are shown at 142dpi Naïve (A), 90dpi Infected Untreated (B), 120 Infected Untreated (C), 142dpi Untreated (D), 120dpi Low BNZ (E), 120dpi Vaccine (F), 120dpi E6020 (G), 142dpi Vaccine + Low BNZ (H), 142dpi Curative BNZ (I). The scale bar represents 100um.
Fig 5.
Impact of infection and treatment on liver enzymes.
Complete serum chemistry analysis was performed. ALT, ALP, AST, and LDH were evaluated because they are the most common liver function tests. *P ≤0.05; **P ≤0.01.
Fig 6.
Infection status and treatments on expression of BTG2 and PPARα.
BTG2 and PPARα were measured in liver tissue. *P<0.05; **P < 0.01; ***P<0.001 ****P < 0.0001.