Fig 1.
Map of Moldova and north-eastern Romania.
The red circles represent the geographical origin of the samples used in the study. The circle sizes correspond to the number of tested samples (enlarge to view). The map depicted in Fig 1 is originally and was created using authorised license of ArcGIS, version 10.5.1.
Table 1.
Characteristics of the brain samples used in the study from Moldova and north-eastern Romania.
Table 2.
Characteristics of primers used in both real-time RT-qPCR and conventional RT-PCR techniques.
Fig 2.
Phylogenetic tree of rabies virus whole genome for Moldova and north-eastern Romania, generated by PhyML and Seaview software.
For Moldova, domestic animals (n = 6) are shown with purple triangles. For north-eastern Romania, domestic animals (n = 4) are shown with blue squares and wild animals (n = 8) with red squares.
Fig 3.
Map of Moldova and north-eastern Romania.
Distribution of the three lineages RO#5, RO#6 and RO#7 in the two countries. RO#5 represented by DR1331 was isolated in a red fox, in 2016, in Vrancea county, Romania. RO#6 is present in both countries, with samples isolated between 2012 and 2016, from different animal species: red fox, cow, goat, ferret, dog, cat and deer. RO#7 included samples from both countries, isolated in 2013, 2014, 2016 and 2017, from red fox, wolf, cat and a cow. The map depicted in Fig 3 is originally and was created using authorised license of ArcGIS, version 10.5.1.
Fig 4.
Bayesian phylogenetic analysis undertaken on partial N gene sequences from Romania and Moldova.