Table 1.
Source of reference strains.
Table 2.
Sequence of primers and probe in this study.
Fig 1.
Scatter diagram of optimal annealing temperature in ddPCR.
Note: the annealing temperatures of C01-C12 are 50.0°C, 50.6°C, 51.5°C, 52.7°C, 53.9°C,55.3°C, 56.7°C, 58.1°C, 59.3°C, 60.5°C, 61.4°C and 62.0°C respectively.
Fig 2.
Scatter diagram of optimal primer and probe concentration.
A: Determination of primer concentration: fixing the probe concentration to 250 nmol/L, and each primer concentration has been marked in the figure. B: Determination of probe concentration: fixing the primer concentration to 800 nmol/L, and each probe concentration has been marked in the figure.
Fig 3.
Gradient dilution amplification diagram of ddPCR B. melitensis16M DNA was sequentially diluted 10 fold from stock solution to 10−7.
Table 3.
Results of ddPCR repeatability test.
Fig 4.
Results of ddPCR specificity test.
Note: 1–9 B. melitensis bv1, 2 and 3, B. abortus bv1, 2 and 3, B. suis bv1, B. canis and B. ovis. 10–15 specific strains: Yersinia enterocolitica O:9, Yersinia enterocolitica O:3, Salmonella typhimurium, Escherichia coli O157, Vibrio parahaemolyticus; Vibrio cholerae O1.
Table 4.
Whole blood samples detected by qPCR and dd PCR.
Table 5.
Changes of Ct values and copies after follow-up treatment.