Fig 1.
Bartonella sp.-PCR results from blood donors represented as Venn diagrams, showing how many samples were amplified in each reaction and detected in more than one PCR.
A: Bartonella sp.-DNA was amplified in 30/500 (6%) blood samples. B: Bartonella sp.-DNA was detected in 77/500 (15.4%) liquid cultures. C: Bartonella sp.-DNA was detected in 102/500 (20.4%) from blood and liquid culture.
Table 1.
Initial amount of Bartonella sp.-DNA genome equivalent (GE) per mL of blood required in the initial sample for amplification.
Table 2.
List of sequenced samples and regions.
Fig 2.
Comparison between results obtained analyzing blood versus liquid culture samples (considering ‘detectable in any PCR’ as the gold standard) by McNemar-Bowker test. PPV: positive predictive value; NPV: negative predictive value.
Fig 3.
Comparison between different PCR tests for B. henselae.
A: Blood samples; B: Liquid culture; C: Combining blood and liquid culture. PPV: positive predictive value; NPV: negative predictive value.
Fig 4.
ROC (receiver operating characteristic) graphical representations generated from the results of different PCR tests for Bartonella henselae considering ‘detectable in any PCR’ as the gold standard.
A: from blood samples; B: from liquid culture samples. AUC: Area under the curve. In the plot, the yellow 45-degree line marks the cutoff point that maximizes the sum of sensitivity and specificity.