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Fig 1.

Rainfall (mm) and temperature (°C) during the year 2019 in Phop Phra district, Tak province in 2019 (A).

The density and distribution of rodent species captured over one year period in Wale sub-district, Phop Phra district, Tak province in 2019 (B).

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Table 1.

prevalence in rodents and their associated ticks (2019–2020).

Borrelia spp.

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Fig 2.

Phylogenetic trees of 16S rRNA gene of Borrelia species detected in rodents and their associated ticks in Phop Phra district, Tak province, Thailand (2019–2020).

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Fig 3.

Phylogenetic trees of flagellin gene of Borrelia species detected in rodents and their associated ticks in Phop Phra district, Tak province, Thailand (2019–2020).

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Table 2.

Sequence identity matrix of Borrelia spp. detected in examined rodents and their associated ticks from Phop Phra District, Tak province (2019–2020).

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Fig 4.

Polyacrylamide gel electrophoresis (SDS-PAGE) of B. miaymotoi recombinant GlpQ protein (A) and Western blot analysis (B) of Tak patient (TAK016) positive for B. miyamotoi by the in-house ELISA assay (titer 400).

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Table 3.

The cross-reactivity of the in-house ELISA assay to vector-borne diseases previously reported in Thailand.

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Fig 5.

The percentage of positive anti-B. miyamotoi rGlpQ IgG antibody in rodents collected every other month in 2019–2020, Phop Phra district, Tak province.

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Table 4.

The serological reactivity to B. miyamotoi rGlpQ protein in rodents captured from Phop Phra district, Tak province (2019–2020).

The serological reactivity as well as rodent IgG antibody titers in each rodent species were shown.

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Table 5.

The serological reactivity of human sera to Borrelia miyamotoi rGlpQ protein in Phop Phra district, Tak province, Thailand.

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