Fig 1.
An overview of the CYP2D6 gene structure and the primers used to detect deletion, multiplication and CYP2D6*36+*10 tandem.
Table 1.
Genotypic analysis of G6PD deficiency.
Table 2.
Kinetic parameters of G6PD variants.
Fig 2.
Biochemical and structural characterization of recombinant G6PD variants.
(A) Far-UV spectra were recorded over a wavelength range of 190 to 260 nm. (B) Susceptibility to trypsin digestion in the presence of various concentrations of NADP+. (C) Stability analysis upon Gdn-HCl treatment in the presence of various concentrations of NADP+. (D) Thermal stability analysis in the presence of various concentrations of NADP+. Error bars represent the mean ± SD of triplicate measurements.
Table 3.
Frequency of CYP2D6 allele and SNPs identified in this study.
Table 4.
Frequency of genotypes and predicted phenotype of CYP2D6.
Fig 3.
Profile of G6PD deficiency and CYP2D6 in the studied population.
The distribution of (A) G6PD genotypic status (n = 88), (B) CYP2D6 functional alleles (n = 176), and (C) predicted CYP2D6 phenotype (n = 88).
Fig 4.
Three-dimensional structure of human G6PD enzyme (PDB: 2BHL and 2BH9).
The structural NADP+, NADP+ coenzyme, and G6P substrate are shown as purple, pink, and yellow molecular surface representations, respectively. The mutants are shown in CPK representation. The graphical representation was constructed using Discovery Studio Visualizer-Accelrys.