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Table 1.

List of mosquito colonies/populations used for YFV infections.

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Fig 1.

Infection rate, dissemination efficiency, and transmission efficiency of Aedes aegypti AAPAEA and Aedes albopictus ALPROV exposed to vYF-247 and S-79 strains.

Mosquitoes were presented to a blood meal provided at a titer of 107.5 ffu/mL and examined at 7, 14, and 21 days post-infection. In brackets, number of tested mosquitoes. Stars indicate statistical significance of comparisons by Fisher’s exact test: *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.

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Fig 2.

Number of viral particles (Log10) in abdomen, HT, and saliva of Aedes aegypti AAPAEA and Aedes albopictus ALPROV exposed to vYF-247 and S-79 strains.

Mosquitoes were presented to a blood meal provided at a titer of 107.5 ffu/mL and examined at 7, 14, and 21 days post-infection. In brackets, number of YFV-positive mosquitoes. Stars indicate statistical significance of comparisons by Kruskal-Wallis test: **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

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Fig 2 Expand

Fig 3.

Infection rate, dissemination efficiency, transmission efficiency, Log10 viral particles in abdomen, HT, and saliva of 4 Aedes aegypti populations exposed to vYF-247 and YFV S-79 strains.

Mosquitoes were presented to a blood meal provided at a titer of 107.5 ffu/mL and examined at 21 days post-infection. In brackets, number of tested mosquitoes for IR, DE, TE, and number of YFV-positive abdomens, HT and saliva. Stars indicate statistical significance of comparisons: *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.

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Fig 3 Expand

Fig 4.

Infection rate, dissemination efficiency, transmission efficiency (a), Log10 viral particles in abdomen, head and thorax, and saliva (b) of Aedes albopictus Tainan (Taiwan) exposed to vYF-247 and S-79 strains.

Mosquitoes were presented to a blood meal at a titer of 107.5 ffu/mL and examined at 21 days post-infection. In brackets, number of tested mosquitoes for IR, DE, TE, and number of YFV-positive abdomens, HT and saliva. Stars indicate statistical significance of comparisons: *p ≤ 0.05, **p ≤ 0.01.

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Fig 4 Expand