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Fig 1.

Geographic location of the nine research clusters in Chiang Mai, Thailand.

Nine research clusters included (1) rural district (n = 3): Wiang Haeng, Mae On, and Galyani Vadhana; (2) urban district (n = 3): Mueang, Saraphi, and San Sai; and (3) peri-urban district (n = 3): Hang Dong, Hot, and Phrao, according to the 2010 population and housing census of Thailand. Note: The base layer of the map used in this figure comes from the Thailand—Subnational Administrative Boundaries of the Royal Thai Survey Department. (https://data.humdata.org/dataset/cod-ab-tha). The map was generated by the Quantum GIS: QGIS Software, version 3.24.3.

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Table 1.

Protocol-defined seroprotection definitions against Japanese encephalitis virus among study participants.

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Table 2.

Characteristics of study participants.

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Fig 2.

Age-stratified seroepidemiology of Japanese encephalitis virus among study participants.

Abbreviations: JEV, Japanese encephalitis virus; PRNT50, 50% plaque reduction neutralization test; PRNT90, 90% plaque reduction neutralization test. Bar chart represents the proportion of study participants with protocol-defined seroprotection against JEV. Line chart represents the neutralizing antibody to JEV based on PRNT50 (dashed line) and PRNT90 (dotted and dashed line). Vertical line represents the 95% confidence interval of each parameter.

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Table 3.

Summary of age-stratified seroepidemiology of Japanese encephalitis virus, based on all three serological definitions against Japanese encephalitis virus.

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Table 4.

Associated factors of protocol-defined seropositivity against Japanese encephalitis virus among study participants, stratified by age group.

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