Fig 1.
Experimental design and timeline.
Bats were inoculated (SC) with 2 X 105 PFUs of ZIKV Mer.IPN01. Groups, sampling, and time of sacrifice A. Histopathological and gross findings B.- No changes observed.+ Mild.++ Moderate.* Urine samples positive for ZIKV RNA by qRT–PCR. Only two urine samples tested positive for ZIKV by RT–qPCR, ranging from 3.19 X 102 3.61 X 102 copies/mL, urine sample from bat MO08 on day 3 p.i. and urine samples from bat MO05 on day 9 p.i. All other samples (urine, swabs, and tissues) tested negative.# control group.
Fig 2.
Sites and years where and when bat samples were obtained.
Note.- The size of the dots is proportional to the number of years sampled.
Table 1.
List of bats sampled in French Guiana, Peru, and Costa Rica from 2010 to 2017 for the detection of ZIKV in blood by RT–PCR and antibodies by seroneutralization test.
Table 2.
Complete blood count.
Fig 3.
Petechiae on the right wing of bat MO06 observed 3 days p.i.
Fig 4.
Photomicrographs of sections of the epididymis.
A. Control epididymal ducts. H&E stain; bar = 50 μm. B. Epididymal duct epithelial degeneration, vacuolization, and loss of stereocilia. The lumen is filled by abundant degenerate to apoptotic spermatids. H&E stain; bar = 50 μm. Photomicrographs of sections of the testicles. C. Control testicular parenchyma. D. Early stage of testicular degeneration, vacuolated Sertoli cells with disorganized exfoliated or absent germ cells. H&E stain; bar = 50 μm. Photomicrographs of sections of the oviduct. E. The lamina propria is infiltrated by scant neutrophils. F. The degenerated epithelium has lymphocytic exocytosis, and the oviduct lumen is filled by granular eosinophilic proteinaceous secretion intermixed with neutrophils. H&E stain; bar = 50 μm.
Fig 5.
Photomicrographs of sections of the cerebellum.
A. Control: molecular, Purkinje cell, and granular layer. H&E stain; bar = 50 μm. Purkinje cells (inset). H&E stain; bar = 50 μm. B. infected: Photomicrographs of sections of the cerebellum. Necrosis of the Purkinje cell layer. H&E stain; bar = 100 μm. Necrotic cells are shrunken, with angulated cellular margins, hypereosinophilic cytoplasm, and pyknotic nuclei (inset). H&E stain; bar = 50 μm.
Fig 6.
Photomicrographs of sections of the patagium.
H&E stain; bar = 200 μm. The dermis has hemorrhages and has a mild interstitial neutrophilic infiltrate (inset). H&E stain; bar = 50 μm.
Fig 7.
Photomicrographs of sections of the spleen.
The white pulp has marked lymphoid follicular hyperplasia. H&E stain; bar = 100 μm. Lymphoid follicles have prominent germinal centers. H&E stain; bar = 50 μm.