Fig 1.
Infection kinetics among primary and post-primary DENV patients from Indonesia by disease day according to laboratory and rapid tests.
A-H: Boxplots of viremia (Ct), NS1, IgM and IgG by disease day among primary and post-primary cases according to PCR and ELISA tests. I-P: Proportion RDT positive to NS1, IgM, IgG and all combined by disease day among primary and post-primary cases. Black error bars: 90% confidence intervals based on t-distributions. (Primary N: 55) (Post-primary N:109).
Fig 2.
Estimated probability of being DENV RDT positive according to corresponding laboratory-derived metrics using logistic regression modelling.
A: IgM RDT positivity according to IgM panbio units and the corresponding sensitivity/specificity among patients from Indonesia (N:200) B: IgG RDT positivity according to IgG panbio units and the corresponding sensitivity/specificity among patients from Indonesia (N:200) C: NS1 RDT positivity according to viremia (Ct value) and the corresponding sensitivity/specificity stratified by disease day among patients from Vietnam (N: 1,217). Grey dash: estimated laboratory-derived metric threshold for RDT positivity according to the optimal Youden’s J index value.
Table 1.
Estimated laboratory-test values that yield RDT positive results compared to actual RDT outcomes.
Estimated RDT positivity threshold refer to the optimal Youden’s J index value. Estimated/actual NS1 RDT positivity determined among patients from Vietnam (N: 1,217). Estimated/actual IgM/IgG RDT positivity determined among patients from Indonesia (N:200).
Table 2.
Agreement between the estimated and actual combined DENV RDT results of patients in Indonesia.
Table 3.
The probability of being primary, post-primary or historical for DENV according to every outcome combination of NS1, IgM and IgG RDTs stratified by disease day.
RDT results estimated among patients from across the Philippines (N: 28,326).