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Fig 1.

Map of the field sites in Florida City, FL, USA (1), and Metetí, Darien Province, Panama (3).

Base layer country maps downloaded from: https://www.diva-gis.org/gdata

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Fig 2.

Illustrations of the six mosquito collection methods used by this study.

Large-diameter aspirator is depicted in Fig 2A–2C. Lateral view of the small-diameter aspirator illustrated in 2d. BG-Sentinel and the CDC-light trap are illustrated in 2e and 2f respectively. The pop-up resting shelter and mosquito drift fence are illustrated in 2g-2h.

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Fig 3.

Weather data during 2019 for Florida and Panama, including precipitation (blue) and temperature (orange). Mosquito sampling periods are highlighted in yellow Panama data is from ETESA and Florida data is from NOAA.

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Fig 4.

Relative proportions of unfed and blood-engorged Spissipes section mosquitoes.

Mosquitoes were collected using the large diameter aspirator (LDA), BG-Sentinel (BGS), CDC miniature light trap (CDC), mosquito drift fence (MDF), pop-up resting shelter (PRS), and the small diameter aspirator (SDA) in Darién, Panama, and Florida City, FL. Chi-squared values represent differences in trap efficacy between unfed and blood-engorged of the same species.

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Fig 5.

Bipartite analysis of the subgenera of Culex collected by each trap type.

Proportions of major subgenera Culex (yellow), Melanoconion (red), and Aedinus (blue) captured by five of the large-diameter aspirator (LDA), BG-Sentinel (BGS), CDC miniature light trap (CDC) (shown for Panama only), mosquito drift fence (MDF), pop-up resting shelter (PRS), and the small-diameter aspirator (SDA) (excluding the CDC trap in Florida only) are depicted for Florida City, FL (A), and Darién, Panama collections (B).

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Table 1.

Number of each mosquito species captured for the large diameter aspirator (LDA), BG-Sentinel (BGS), CDC miniature light trap (CDC), mosquito drift fence (MDF), pop-up resting shelter (PRS), and the small diameter aspirator (SDA) in Florida City, Florida.

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Table 2.

Number of each mosquito species captured for the large diameter aspirator (LDA), BG-Sentinel (BGS), CDC miniature light trap (CDC), mosquito drift fence (MDF), pop-up resting shelter (PRS), and the small diameter aspirator (SDA) in Darién, Panama.

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Table 3.

Values for species richness, Simpson index, Shannon index, and Pielou’s index for the large diameter aspirator (LDA), BG-Sentinel (BGS), CDC miniature light trap (CDC), mosquito drift fence (MDF), pop-up resting shelter (PRS), and the small diameter aspirator (SDA) in Florida and Panama.

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Fig 6.

Species accumulation curves of mosquito species collected by the large diameter aspirator (LDA), BG-Sentinel (BGS), CDC miniature light trap (CDC), mosquito drift fence (MDF), pop-up resting shelter (PRS), and the small diameter aspirator (SDA) in Florida and Panama.

Species accumulation curves of mosquito species captured by the six traps used in Florida City, Florida (A), and Darién, Panama (B). Pie charts correspond to the distribution of mosquito genera captured in each trap.

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