Fig 1.
A) Field sites located in the periphery of the city of Merida, southeast of Mexico; San Pedro Chimay (release site) shown in orange and Tahdzibichén (control site) in blue; B) Distribution of one-hectare areas within localities, where sentinel areas for entomological surveillance were located and releases were performed (only at San Pedro Chimay); C) Proposed an Integrated Vector Management plan combining ‘traditional Aedes control’ and the release of X-ray irradiated male Ae. aegypti carrying Wolbachia for population suppression structured in three phases: Preparation: with community sensitization and engagement, and baseline entomological studies; Attack: initial traditional vector control; and Suppression phase: with inundative releases during peak of mosquito abundance; D) Sterile male mosquitoes released in a backyard by personnel of the MoH; E) "Uts koxol” (“good mosquitoes” in Mayan language). Maps were produced using QGIS based on public geographic data obtained from OpenStreetMap (www.openstreetmap.org).
Fig 2.
A) Laboratory of Biological Control for Aedes aegypti (LCB-UADY). B) Schematic representation of the main processes at LCB-UADY to produce wAlbB Ae. aegypti mosquitoes with the sterile insect technique using radiation (IIT-SIT). As part of the Q.C., we optimized a PCR assay to detect the presence of Wolbachia infection in every generation of adult mosquitoes under mass-rearing system as well as before every release.
Fig 3.
Entomological indicators of population suppression between release (San Pedro Chimay = red line) and control (Tahdzibichén = black line) sites.
A) Mean hatching rate (SD) per week, B) Mean No. of females captured with BG traps (SD), C) Mean No. of indoor females captured with Prokopack aspirators, D) Average temperature and precipitation during the study period. Grey-shaded regions indicate release periods. The dashes in the X-axis represent the weeks of the year.
Table 1.
Analyses of entomological indicators of population suppression between release (San Pedro Chimay) and control (Tahdzibichén) sites.
Incidence rate ratios (IRR) and odds ratios (OR) with 95% confidence intervals are shown. Intervention efficacy ([1- OR or 1-IRR] x100) is shown.
Table 2.
Cost estimates table (USD) for the implementation of ULV spraying and release of IIT-SIT wAlbB males identified for an area of 50 ha v.gr.
San Pedro Chimay.