Fig 1.
Geographic location of Iguaçu National Park, Brazil, points of capture and number of wild carnivores and domestic dogs sampling.
(Red diamonds represent the sites of captures of wild carnivores; Blue circles represent the sites of domestic dogs samplings in rural areas; Yellow star represents the site of domestic dogs sampling in urban area) Source of base layer https://www.openstreetmap.org/#map=9/-25.6613/-54.7833.
Table 1.
Source of the studied wild carnivores and type of collected sample from PARNA Iguaçu.
Table 2.
Primers designed for amplification of myoHC and hsp70 genes of selected Onchocercidae species.
Fig 2.
Morphology of the microfilariae detected in ring-tailed coatis (Nasua nasua) and domestic dogs from the studied area.
1. Mansonella sp. 1: A- Anterior and posterior; 2. Mansonella sp. 2: B—Anterior and posterior; 3. Onchocercidae gen. sp. 1—C: anterior and posterior; 4. Onchocercidae gen. sp. 2—D: Anterior and posterior; 5. Acanthocheilonema reconditum—E: anterior and posterior. 100x magnification light microscopy.
Fig 3.
Bayesian phylogenetic tree of myoHC gene sequences of Onchocercidae microfilariae haplotypes present in blood samples of ring-tailed coatis (Nasua nasua) and domestic dog from the Iguaçu National Park.
Isolates from this study are highlighted. The blue branches are related to Mansonella spp clade, the cyan branches are related to the sheated species clade, the red branches represent Dirofilaria immitis clade, and the magenta branches represent Acanthocheilonema reconditum.
Fig 4.
Bayesian phylogenetic tree of hsp70 gene sequences of Onchocercidae microfilariae haplotypes present in blood samples of ring-tailed coatis (Nasua nasua) and domestic dog from the Iguaçu National Park.
Isolates from this study are highlighted. The blue branches are related to Mansonella spp clade, the red branches represent Dirofilaria immitis clade, the magenta branches represent Acanthocheilonema reconditum, and the the green branches are related to the sheated species clade.
Fig 5.
Spatial distribution of the ring-tailed coatis (Nasua nasua) and domestic dogs infected by Onchocercidae nematodes, PARNA Iguaçu.
Source of base layer https://www.openstreetmap.org/#map=9/-25.6613/-54.7833.
Table 3.
Prevalence of Onchocercidae nematodes in ring-tailed coatis and domestic dogs from the Iguaçu National Park, in relation to the diagnostic tests.