Table 1.
Characteristics and number of tested serum samples.
Fig 1.
Integrity and concentration of the recombinant proteins.
Lane Mk: Biorad Precision plus protein dual color marker standards. Lanes 1–7: Bovine serum albumin (BSA) protein standard curve in decreasing concentrations. Lane 1: 1 mg /mL. Lane 2: 0.5 mg /mL. Lane 3: 0.25 mg /mL. Lane 4:0.125 mg /mL Lane 5: 0.062 mg /mL. Lane 6: 0.031 mg /mL Lane 7: 0.015 mg /mL. Lane 8: 1/20 dilution of recombinant protein T24H. Lane 9: undiluted recombinant protein 2B2t.
Fig 2.
ROC curves analysis of different tests.
A.ROC curves analysis showing comparison of rT24H (MBA) and Novalisa (ELISA) with all serum samples sets. B.ROC curves analysis showing comparison of r2B2t (MBA) and Ridascreen (ELISA) with all serum samples sets. Abbreviations: χ2:Chi-Square; SE: Standard error; CI: confidence interval; ROC: Receiver Operating Characteristic; AUC: Area under the ROC curve.
Table 2.
Diagnostic test accuracy of rT24H and r2B2t in MBA.
Table 3.
Equivocal results.
Table 4.
Diagnostic accuracy of rT24H- and r2B2t- MBA, and Novalisa, Ridascreen ELISA assays when excluding equivocal ELISA results from the analysis.
Table 5.
Diagnostic accuracy of rT24H- and r2B2t- MBA, and Novalisa, Ridascreen ELISA assay when ELISA equivocal results were considered as positive or negative.